Multiresidue analysis of 85 persistent organic pollutants in small human serum samples
On this examine, analytical strategies multiresidue developed, validated, and utilized to measure 85 persistent pollution natural (POPs), together with 38 polychlorinated biphenyls (PCBs), 23 polybrominated diphenyl ether (PBDE), and 24 organochlorine pesticides (OCPs) of 200 uL serum human.
A modified QuEChERS (Fast, Simple, Low cost, Efficient, Rugged, and Secure) technique is utilized to attenuate the variety of samples required and optimize varied circumstances together with solvent extraction and extraction quantity. Extraction effectivity is optimized utilizing a double extraction with ethyl acetate / hexane / acetone combination. fuel chromatography coupled with triple-quadrupole mass spectrometry is used for evaluation, and two completely different sources of ionization, the electron impression ionization (EI) and atmospheric strain chemical ionization (APCI), is used to check the sensitivity of them.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.
Description: A sandwich ELISA for quantitative measurement of Mouse Total prostate s pecific antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Total prostate s pecific antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Total prostate s pecific antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
1 kit (48 extractions) Including all required plastic disposables
EUR 369.6
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Prostate Specific Antigen (Total, human) ELISA Kit
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Mouse Normal Tissue: Skeletal Muscle
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Mouse Normal Tissue: Small Intestine
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from hypertension: Interventricular Septum
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
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APCI supply employed comfortable ionization at atmospheric strain, ensuing within the formation of molecular ions abound with minimal fragmentation, in contrast to the intensive fragmentation attributable to EI. Of the 85 POPs analyzed, the goal compound 59 (69.4%) confirmed a decrease restrict of detection of two to fifty-fold decrease in APCI than that decided utilizing EI. Superior technique is validated for the detection restrict (0.5 to 10 pg / mL for PCB, 2-20 pg / mL for PBDEs, and 2-40 pg / mL for OCP), precision (0.8% -34.3% of coefficient of variation), restoration (49.6% -77.1%), matrix results (46.7% -156.9%) and accuracy (81.2% -113.1% for PCBs, 85.8% -112 , 2% to PBDE, and 55.2% -113.9% for OCP).
linearity is R2> 0.99 for 84 compounds, and 96% common accuracy (for APCI) have been obtained utilizing Nationwide Institute of Know-how (NIST) requirements and commonplace reference supplies (NIST 1957 and 1958). This ionization technique in contrast by analyzing 25 actual human serum samples. The species noticed have been 1.1 to 24.6 pg / mL 28 PCB, 2.5 pg / mL BDE-47, and 6.5 to 195.1 pg / mL 6 organochlorine pesticides (common focus for every species) and solely 11 compounds have been detected by APCI for enhanced sensitivity.
Multiresidue evaluation of 85 persistent natural pollution in small human serum samples by modified QuEChERS preparation with completely different ionization sources in mass spectrometry
Finest Practices within the Use of 2D-NMR spectral evaluation of information as enter for Biopharmaceutical Functions Chemometric.
high quality attributes (QAs) is measurable parameters of the organic impression and effectiveness of product security and important traits related to optimistic affected person well being outcomes. One QA, larger construction (HOS), instantly coupled to the organic features of proteins, and irregularities on this QA may cause hostile results. To deal with the vital want for the evaluation of the HOS, the strategy for analyzing the structural fingerprints of 2D nuclear magnetic resonance spectroscopy (2D-NMR) spectrum has been arrange for a significant drug substances similar to monoclonal antibody remedy.
Right here, the chemometric evaluation has been utilized to 2D 1H, 13C-NMR correlation spectrum of methyl NIST IgG1κ monoclonal antibodies (NISTmAb), famous the abundance of pure isotopes, to benchmark the efficiency and robustness of strategies. Specifically, varied attainable schemes enter spectral (eg, chemical shift, the height depth and the quantity of matrix spectral) into the algorithm chemometric have been examined utilizing two case research: (1) world 2D-NMR research between main laboratories and (2) sequence of combined enzymatic glycan-renovated isoform NISTmAb.
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa
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This case examine reveals {that a} good efficiency chemometric algorithms use the highest or the overall spectral matrix as enter will depend upon the design of the examine and are more likely to be product-specific. Basically, the height place is discovered to be extra highly effective spectral parameters for enter into the chemometric algorithms, whereas the overall spectral matrix method is appropriate for automation simpler and requires much less consumer intervention.
